EFFECTS OF DIFFERENT PARAMETERS ON THE LIPOLYTIC ACTIVITIES OF LIPASE AND PHOSPHOLIPASE EXTRACTED FROM PRUNUS ARMINICA MEAL OF HUNZA CULTIVAR


 Prunus Arminica.L seeds of Hunza cultivar were defatted with chloroform, methanol mixture (2:1v/v) by ultrasonic assisted extraction technique. The lipase and phospholipase extracted, incubated at 400C pH 6 in citrate buffer for 1 hour. lipolytic activities enzymes measured different temperatures i,e 30-700C , 3-9 solvents n-heptane Di-isopropyl ether Cyclo hexane. 10 % emulsion lecithin olive oil used as the substrate substrates respectively. showed stability lower 35-500C slightly acidic to neutral i.e 5 7. Concentration liberated fatty acids was indicator activity which taking absorbance 440nm. Stearic acid concentrations hexane (1:1v/v) standard curve. Cu-TEA reagent small quantity CaCl2 maximum Cu-FFA stable value blanks present study. 450 C temperature pH.